uid=MCM,o=EDI,dc=edirepository,dc=org all public read sean Seasonal Effects on Anhydrobiosis of Nematodes Diana Wall
Johnson Hall 107 Fort Collins CO 80523 US
Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235
Ross Virginia
Hinman Box 6182 Hanover NH 03755 US
(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981
McMurdo Dry Valleys LTER http://mcmlter.org/ Inigo San Gil
Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US
(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager
2014-01-01 English
Investigation of the effect of long-term variation in soil moisture and soil temperature on nematode anhydrobiosis as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The percent of anhydrobiotic (coiled) nematodes with relation to season was determined. The study took place on three separate dates during the 1997-1998 austral summer: 21 November 1997, 16 December 1997, and 13 January 1998.
nematodes LTER Controlled Vocabulary Anhydrobiosis Antarctica LTER nematodes Station Keywords population dynamics LTER Core Areas
Data Policies This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-1637708). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.
https://mcm.lternet.edu/content/seasonal-effects-anhydrobiosis-nematodes Four contingent polygons were selected in a clockwise direction. The middle of the south facing crack was selected as the "0" point and soil samples were taken at 0, 0.2, 0.5, 2, and 5 m along a transect towards the middle of the polygon (in a south to north direction). This corresponds to the Hoare basin plot. 162.866668701172 162.866668701172 -77.616668701172 -77.616668701172 1997-11-21 1998-01-13 Enhanced in 2016 using DEIMS, Inigo San Gil This file was created by Pilar Tillberg on 10 May 2001, using raw data from the Excel workbook '9711sean.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. [PT 10 May 2001]. McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER
   Sampling was done by using a clean plastic scoop and collecting soil to a 10-cm depth. The soil was scooped onto a 2mm screen and then sifted into a clean metal pan. After sifting, the rocks were discarded and the screen was removed. 100mL of the soil was then poured through a funnel into a 500mL Nalgene bottle containing 100mL of 1.25M sugar solution. The bottle was tightly capped for transportation back to the lab.     The soils were processed within 3 days of collection from the field. Until the n, the soils were stored in a 4_C refrigerator.  To extract the nematodes, first the bottles were filled with an additional 100 mL of 1.25M sugar solution. Then, a long spatula was used to stir the soil so that it was suspended in the sugar (30 seconds of stirring). Next, the sample was poured onto a set of No. 40 over No. 400 U.S.A. standard testing sieves that had been pre-wetted with 1.25M sugar solution. The top screen was rinsed with 1.25M sugar solution and then removed.  The bottom screen was rinsed with 1.25M sugar solution that was squirted only on the front of the screen at an angle. Using this rinsing method, the soil was carefully worked to the bottom wedge of the screen, and the sugar volume was reduced by gently tapping the screen and letting more solution pass through.  Next, the sample was rinsed into a 150mL beaker through a funnel, and then the funnel rinsed to catch any residue.   Once the sample was collected into the 150mL beaker, the sugar and sediment was pipetted (using an automatic pipette) into a centrifuge tube containing 2M sugar solution. The pipetting was done slowly and at an angle so that the boundary between the two sugar layers was maintained. Then the beaker was rinsed wi th 1.25M sugar solution and the dregs were pipetted into the centrifuge tube as well. When necessary, more than one centrifuge tube was used to collect all the sample.   Next, the tubes with sample were centrifuged for five minutes at 1744 RPM. Then, the liquid contents of the tube were poured through a No. 500 sieve. Finally, the contents of the sieve were rinsed into glass centrifuge tubes and stored at 4_C until counting. Samples were washed in to a counting dish and examined under a microscope at x100 or x200 magnification. Nematodes were identified as coiled or straight and counted. Total numbers in each sample were recorded on data sheets. Data were entered in to Excel files, printed, and checked for errors.  
sean Definitions of variables and units used in the dspreadsheet for the SEAN data. sean.csv 4046 1 0 \r\n column , " https://mcm.lternet.edu/sites/default/files/sean.csv LOCATION LOCATION Name of area where measurement was made string Name of area where measurement was made DATE_TIME DATE_TIME Date on which sample was gathered date mm/dd/yyyy SAMPLE # SAMPLE # ID associated with transect, sampling location string ID associated with transect, sampling location TYPE OF ORGANISM TYPE OF ORGANISM Family associated with organism string Nematode a nematode SPECIES SPECIES Species found in soil string Combined Combined - Scottnema, Eudorylaimus, Plectus SEX SEX Gender of organism (male vs. female) string Gender of organism (male vs. female) LIVE/DEAD/COMBINED LIVE/DEAD/COMBINED Survival Status (living, dead, or both) string Combined Live and dead combined ADULT/JUVENILE/COMBINED ADULT/JUVENILE/COMBINED Maturity (adult, juvenile, or both) string Combined Adults and Juveniles PERCENT COILED PERCENT COILED NEMATODES Percentage of the nematodes found in a coiled state dimensionless real Null None given COMMENTS Comments Comments about the overall data string Comments about the overall data FILE NAME FILE NAME Name of file in which data was stored string Name of file in which data was stored