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dpwo9394
Soil Depth Effects on Soil Biota
Diana
Wall
Johnson Hall 107
Fort Collins
CO
80523
US
Diana.Wall@colostate.edu
http://wp.natsci.colostate.edu/walllab/
https://orcid.org/0000-0002-9466-5235
Ross
Virginia
Hinman Box 6182
Hanover
NH
03755
US
(603) 646-0192
ross.a.virginia@dartmouth.edu
http://sites.dartmouth.edu/ravirginia/
https://orcid.org/0000-0002-0890-0981
McMurdo Dry Valleys LTER
http://mcmlter.org/
Byron
Adams
byron_adams@byu.edu
https://biology.byu.edu/adams-lab
https://orcid.org/0000-0002-7815-3352
associated researcher
Andy
Parsons
Natural Resource Ecology Laboratory
Fort Collins
CO
80523
US
andy@nrel.colostate.edu
lab crew
Denise
Steigerwald
data manager
Inigo
San Gil
Department of Biology, MSC03 2020 University of New Mexico
Albuquerque
NM
87131
US
(505) 277-2625
(505) 277-2541
isangil@lternet.edu
data manager
2014-11-11
English
Investigation of the effect of soil depth on soil biota and properties was part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The number of soil organisms (nematodes, rotifers and tardigrades), divided by species, sex and maturity was monitored at various soil depths in Taylor Valley in order to accomplish this. The study began in the austral summer of 1993/1994. Samples were taken on 20-Dec-1993, 6-Jan-1994, 21-Nov-1994 and 26-Dec-1994.
Antarctica
depth
LTER
nematodes
rotifers
soil
Station Keywords
population dynamics
LTER Core Areas
Data Policies
This data package is released under the Creative Commons Attribution 4.0 International License (CC BY 4.0; http://creativecommons.org/licenses/by/4.0/), which allows consumers (hereinafter referred to as “Data Users”) to freely reuse, redistribute, transform, or build on this work (even commercially) so long as appropriate credit is provided. Accordingly, Data Users are required to properly cite this data package in any publications or in the metadata of any derived products that result from its use (in whole or in part). A recommended citation is provided on the summary metadata page associated with this data package in the McMurdo Dry Valleys LTER Data Catalog (https://mcmlter.org/data), and a generic citation may be found on the summary metadata page in the repository where this data package was obtained. When these data contribute significantly to the contents of a publication, Data Users must also acknowledge that data were provided by the NSF-supported McMurdo Dry Valleys Long Term Ecological Research program (OPP-1637708). This data package has been released in the spirit of open scientific collaboration. Hence, Data Users are strongly encouraged to consider consultation, collaboration, and/or co-authorship (as appropriate) with the data package creator(s). Data Users should be aware these data may be actively used by others for ongoing research; thus, coordination may be necessary to prevent duplicate publication. Data Users should also recognize that misinterpretation of data may occur if they are used outside the context of the original study. Hence, Data Users are urged to contact the data package creator(s) if they have any questions regarding methodology or results. While substantial efforts are made to ensure the accuracy of this data package (with all its components), complete accuracy cannot be guaranteed. Periodic updates to this data package may occur, and it is the responsibility of Data Users to check for new versions. This data package is made available “as is” and comes with no warranty of accuracy or fitness for use. The creator(s) of this data package and the repository where these data were obtained shall not be liable for any damages resulting from misinterpretation, use, or misuse of these data. Finally, as a professional courtesy, we kindly request Data Users notify the primary contact referenced in the metadata when these data are used in the production of any derivative work or publication. Notification should include an explanation of how the data were used, along with a digital copy of the derived product(s). Thank you.
https://mcm.lternet.edu/content/soil-depth-effects-soil-biota
Location at the north shoreof Lake Hoare
162.889100000000
162.889100000000
-77.624600000000
-77.624600000000
1993-01-01
1994-12-31
Enhanced in 2016 using DEIMS, Inigo. This file was created by Mark St. John on 14 Oct 1998, using raw data from the Excel workbooks '9312dpso.raw', '9411dpso.raw', and '9412dpso.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. On 19 Oct 1998, the file was submitted to Denise Steigerwald, the MCM LTER data manager, located at INSTAAR, University of Colorado. Upon arrival at INSTAAR, the data manager combined the 3 data files, removed columns for latitude and longitude, and updated the location names to match those provided in the "soil measurement locations" file (from which latitude and longitude can be found). In addition, a column for depth range was added in order to make it possible to compare soil moisture, chlorophyll-a, and soil nematode densities found at a given depth, but stored in different data files (dpca94, dpah94, dpso9394, and dpwo9394 files). The resulting file was reformatted to present in ascii, comma delimited text and MS-DOS text (table layout) on the MCM LTER web site. Both of these files are linked to this web page above. On 28-Oct-1998, Andy Parsons discovered an error in the documentation of the storage temperature used for the soil samples collected. He informed the data manager of this error, resulting in an update from -8C to +5C in the methods portion of the page above.
McMurdo Dry Valleys LTER
http://mcmlter.org/
McMurdo Dry Valleys LTER
http://mcmlter.org/
McMurdo Dry Valleys LTER
Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile 'Whirlpak' bag and clean plastic scoop per sample. Centers of 8 polygons were found and labelled "A" to "H". Samples were taken from within a 10 cm diameter circular area of each plot. The location of the sampling was recorded each year so that areas were not re-sampled. A hole approximately 20 cm deep was dug at the center of each polygon. Samples were taken at depths 0-2.5 cm, 2.5-5 cm, 5-10 cm and 10-20 cm by digging in from the sides. Very large rocks (>20 mm diameter) were excluded from the sample. The soil was shoveled into the 'Whirlpak' bag until three quarters full (about 1.5 kg soil). The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible. The soil samples were stored in a cooler for transportation. On return to the laboratory (within 8 hours of sampling), the soils were stored at +5 degrees C until further processing. In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination. The Whirlpak bags of soil were mixed thoroughly prior to opening. Approximately 200cm3 of soil was placed in a pre-weighed 800mL plastic beaker. Rocks greater than 3-4mm in diameter were removed from the sample. A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum dish, and weighed on a balance accurate to 0.01g. This sample was dried at 105 degrees C for 24 hours. The sample was removed, placed in desiccator to cool down, and re-weighed. These data were used to calculate water content of the soil and to express data as numbers of soil organisms per unit dry weight of soil. The remaining soil in the plastic beaker was weighed. Cold tap water was added up to 650 mL. The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula. Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh. The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through. The water was kept on ice at all times. The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind. The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen. The side of the screen was tapped gently to filter all the water through. The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen. The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently. The suspension was centrifuged for five minutes at 1744 RPM. The liquid was decanted, leaving a few mL on top of the soil particles. The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL. This was stirred gently with a spatula until the pellet was broken up and suspended. The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 5 degrees C until counted. Samples were washed into a counting dish and examined under a microscope at x10 or x20 magnification. Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted. Total numbers in each sample were recorded on data sheets. All species of nematode, and all rotifers and tardigrades found in the sample were recorded. Data were entered in to Excel files, printed, and checked for errors.
DPWO
Definition of variables and units used for the spreadsheet containing the DPWO data
DPWO.csv
412296
1
column
,
https://mcm.lternet.edu/sites/default/files/data/DPWO.csv
DATASET_CODE
DATASET_CODE
Dataset Code used to identify the data in the databases.
string
Dataset Code used to identify the data in the databases.
LOCATION
LOCATION
Location where data was collected.
string
Location where data was collected.
DATE_TIME
DATE_TIME
Date when samples were collected
date
MM/DD/YYYY
DEPTH_RANGE_CM
DEPTH_RANGE_CM
Range of depths where samples were collected in centimeters
string
Range of depths where samples were collected in centimeters
SAMPLE_NUMBER
SAMPLE_NUMBER
alphanumeric identifier for the sample
string
alphanumeric identifier for the sample
TYPE_OF_ORGANISM
TYPE_OF_ORGANISM
The type of organism
string
Nematode
an antarctic nematode
Rotifer
a rotifer
Tardigrades
A tardigrade
SPECIES
SPECIES
The species
string
Eudorylaimus
Eurorylaimus
Plectus
Plectus
Scottnema
Scottnema
Combined
A mix
Unknown
Not known
SEX
SEX
SEX of the organism
string
Male
male
Female
female
Combined
A mix
Undetermined
Unknown or undetermined
LIVE_DEAD_COMBINED
LIVE_DEAD_COMBINED
Living status
string
Live
Alive
Dead
Dead
Combined
Mix of dead and alive
Undetermined
Cannot or did not determine living status
ADULT_JUVENILE_COMBINED
ADULT_JUVENILE_COMBINED
Lifecycle stage
string
Adult
Adult stage
Juvenile
Juvenile stage
Combined
Mix of adult and juvenile
Undetermined
Did not determine stage
TOTAL_NUM_PER_KG_DRY_SOIL
TOTAL_NUM_PER_KG_DRY_SOIL
biomass in numbers per kilogram of dry soil
numberPerKilogram
real
FILE_NAME
FILE_NAME
The provenance file name
string
The provenance file name