uid=MCM,o=EDI,dc=edirepository,dc=org all public read dpwo9394 Soil Depth Effects on Soil Biota Diana Wall
Johnson Hall 107 Fort Collins CO 80523 US
Diana.Wall@colostate.edu http://wp.natsci.colostate.edu/walllab/ https://orcid.org/0000-0002-9466-5235
Ross Virginia
Hinman Box 6182 Hanover NH 03755 US
(603) 646-0192 ross.a.virginia@dartmouth.edu http://sites.dartmouth.edu/ravirginia/ https://orcid.org/0000-0002-0890-0981
McMurdo Dry Valleys LTER http://mcmlter.org/ Byron Adams byron_adams@byu.edu https://biology.byu.edu/adams-lab https://orcid.org/0000-0002-7815-3352 associated researcher Andy Parsons
Natural Resource Ecology Laboratory Fort Collins CO 80523 US
andy@nrel.colostate.edu lab crew
Denise Steigerwald data manager Inigo San Gil
Department of Biology, MSC03 2020 University of New Mexico Albuquerque NM 87131 US
(505) 277-2625 (505) 277-2541 isangil@lternet.edu data manager
2014-11-11 English
Investigation of the effect of soil depth on soil biota and properties was part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The number of soil organisms (nematodes, rotifers and tardigrades), divided by species, sex and maturity was monitored at various soil depths in Taylor Valley in order to accomplish this. The study began in the austral summer of 1993/1994. Samples were taken on 20-Dec-1993, 6-Jan-1994, 21-Nov-1994 and 26-Dec-1994.
Antarctica depth LTER nematodes rotifers soil Station Keywords population dynamics LTER Core Areas
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https://mcm.lternet.edu/content/soil-depth-effects-soil-biota Location at the north shoreof Lake Hoare 162.889100000000 162.889100000000 -77.624600000000 -77.624600000000 1993-01-01 1994-12-31 Enhanced in 2016 using DEIMS, Inigo.   This file was created by Mark St. John on 14 Oct 1998, using raw data from the Excel workbooks '9312dpso.raw', '9411dpso.raw', and '9412dpso.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. On 19 Oct 1998, the file was submitted to Denise Steigerwald, the MCM LTER data manager, located at INSTAAR, University of Colorado.                            Upon arrival at INSTAAR, the data manager combined the 3 data files, removed columns for latitude and longitude, and updated the location names to match those provided in the "soil measurement locations" file (from which latitude and longitude can be found). In addition, a column for depth range was added in order to make it possible to compare soil moisture, chlorophyll-a, and soil nematode densities found at a given depth, but stored in different data files (dpca94, dpah94, dpso9394, and dpwo9394 files). The resulting file was reformatted to present in ascii, comma delimited text and MS-DOS text (table layout) on the MCM LTER web site. Both of these files are linked to this web page above.                          On 28-Oct-1998, Andy Parsons discovered an error in the documentation of the storage temperature used for the soil samples collected. He informed the data manager of this error, resulting in an update from -8C to +5C in the methods portion of the page above.   McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER http://mcmlter.org/ McMurdo Dry Valleys LTER
   Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile 'Whirlpak' bag and clean plastic scoop per sample.  Centers of 8 polygons were found and labelled "A" to "H".  Samples were taken from within a 10 cm diameter circular area of each plot.  The location of the sampling was recorded each year so that areas were not re-sampled.  A hole approximately 20 cm deep was dug at the center of each polygon.  Samples were taken at depths 0-2.5 cm, 2.5-5 cm, 5-10 cm and 10-20 cm by digging in from the sides.  Very large rocks (>20 mm diameter) were excluded from the sample.  The soil was shoveled into the 'Whirlpak' bag until three quarters full (about 1.5 kg soil). The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible.  The soil samples were stored in a cooler for transportation.  On return to the laboratory (within 8 hours of sampling), the soils were stored at +5 degrees C until further processing.     In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination.  The Whirlpak bags of soil were mixed thoroughly prior to opening.  Approximately 200cm3 of soil was placed in a pre-weighed 800mL plastic beaker.  Rocks greater than 3-4mm in diameter were removed from the sample.  A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum dish, and weighed on a balance accurate to 0.01g.  This sample was dried at 105 degrees C for 24 hours.  The sample was removed, placed in desiccator to cool down, and re-weighed.  These data were used to calculate water content of the soil and to express data as numbers of soil organisms per unit dry weight of soil.       The remaining soil in the plastic beaker was weighed.  Cold tap water was added up to 650 mL.  The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula.  Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh.  The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through.  The water was kept on ice at all times.  The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind.  The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen.  The side of the screen was tapped gently to filter all the water through.  The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen.  The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently.  The suspension was centrifuged for five minutes at 1744 RPM.  The liquid was decanted, leaving a few mL on top of the soil particles.  The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL.  This was stirred gently with a spatula until the pellet was broken up and suspended.  The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 5 degrees C until counted.     Samples were washed into a counting dish and examined under a microscope at x10 or x20 magnification.  Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted.  Total numbers in each sample were recorded on data sheets.  All species of nematode, and all rotifers and tardigrades found in the sample were recorded.  Data were entered in to Excel files, printed, and checked for errors.  
DPWO Definition of variables and units used for the spreadsheet containing the DPWO data DPWO.csv 412296 1 column , https://mcm.lternet.edu/sites/default/files/data/DPWO.csv DATASET_CODE DATASET_CODE Dataset Code used to identify the data in the databases. string Dataset Code used to identify the data in the databases. LOCATION LOCATION Location where data was collected. string Location where data was collected. DATE_TIME DATE_TIME Date when samples were collected date MM/DD/YYYY DEPTH_RANGE_CM DEPTH_RANGE_CM Range of depths where samples were collected in centimeters string Range of depths where samples were collected in centimeters SAMPLE_NUMBER SAMPLE_NUMBER alphanumeric identifier for the sample string alphanumeric identifier for the sample TYPE_OF_ORGANISM TYPE_OF_ORGANISM The type of organism string Nematode an antarctic nematode Rotifer a rotifer Tardigrades A tardigrade SPECIES SPECIES The species string Eudorylaimus Eurorylaimus Plectus Plectus Scottnema Scottnema Combined A mix Unknown Not known SEX SEX SEX of the organism string Male male Female female Combined A mix Undetermined Unknown or undetermined LIVE_DEAD_COMBINED LIVE_DEAD_COMBINED Living status string Live Alive Dead Dead Combined Mix of dead and alive Undetermined Cannot or did not determine living status ADULT_JUVENILE_COMBINED ADULT_JUVENILE_COMBINED Lifecycle stage string Adult Adult stage Juvenile Juvenile stage Combined Mix of adult and juvenile Undetermined Did not determine stage TOTAL_NUM_PER_KG_DRY_SOIL TOTAL_NUM_PER_KG_DRY_SOIL biomass in numbers per kilogram of dry soil numberPerKilogram real FILE_NAME FILE_NAME The provenance file name string The provenance file name