rsan
English
utf8
dataset
McMurdo Dry Valleys LTER
http://mcmlter.org/
2014-10-31
ISO 19115-2 Geographic Information - North American Profile Metadata - Data with Biological Extensions
ISO 19115-2:2009(E)
Stream Transects - Anhydrobiosis of Nematodes
2014-10-31
publication
Diana Wall
Colorado State University
http://www.colostate.edu
Johnson Hall 107
Fort Collins
CO
80523
US
Diana.Wall@colostate.edu
http://wp.natsci.colostate.edu/walllab/
pointOfContact
documentDigital
Investigation of the effect of hydrological and biogeochemical linkages in the transition zone between streams and surrounding soils on invertebrate community structure as part of the McMurdo Dry Valleys Long Term Ecological Research (LTER) project. The invertebrate community structure with relation to soil moisture, salinity, and chlorophyll-a was determined. The study took place on 28 November 1997 and 31 December 1997 at the Von Guerard Stream/Harnish Creek network.
Name: Ross A. Virginia Role: associated researcher Name: John "Jeb" E. Barrett Role: field crew Name: Byron J. Adams Role: field crew Name: Inigo San Gil Role: data manager
completed
McMurdo Dry Valleys LTER
http://mcmlter.org/
unknown
Metadata completed in 2016. This file was created by Pilar Tillberg on 11 May 2001, using raw data from the Excel workbook '9711rsan.raw'. The file format was suggested by the LTER data manager, to conform with the relational database structure. [PT 11 May 2001].
population dynamics
theme
LTER Core Areas
English
Transect by Von Guerard Stream at Upper Site Moss Site M-05 Transect 5 Points T051 and T052 mark the start and end of the transect. Start : IDs: 5VGUPPERT051 Ellipsoid: 114.08 ORTHOMETRIC HT (m) 169.27 End: ID: 5VGUPPERT052 Ellipsoid: 120.24 ORTHOMETRIC HT (m) 175.41 File provenance: strm-clg.txt
163.307327270508
163.305282592773
-77.634559631348
-77.634208679199
Transect by Von Guerard Stream at gage Moss Site M-12, F12Vong-G Transect 12 Points T121 and T122 mark the start and end of the transect. Start : IDs: 12VGGAGET121, Ellipsoid: -30.67 ORTHOMETRIC HT (m) 24.53 End: ID: 12VGGAGET122 Ellipsoid: -31.35 ORTHOMETRIC HT (m) 23.87 File provenance: strm-clg.txt
163.255294799805
163.253005981445
-77.609474182129
-77.609222412109
USGS site 5; coordinates taken from 1996-97 GPS measurements at center of weir Parent Stream: Von Guerard Stream Provenance : GPS96-97.DOC ID: vguerard_f6
163.253997802734
163.253997802734
-77.608200073242
-77.608200073242
Von Guerard Stream at F21. Id: uvg_f21.This was an ill-fated upstream gage on Von Guerard, 2.5 kilometers upstream from F6. It was installed during the lowest season of record (00-01) and then promptly destroyed during the 01-02 season. It's parts were stripped and it never generated a hydrology database, but was certainly sampled.
163.301254272461
163.301254272461
-77.626380920410
-77.626380920410
On the Stream team data page
163.302963256836
163.302963256836
-77.632797241211
-77.632797241211
Parent Stream: Harnish Creek Tributary, also known as Relict Channel
163.294113159180
163.294113159180
-77.633735656738
-77.633735656738
ground condition
1997-11-28
1997-12-31
https://mcm.lternet.edu/sites/default/files/data/rsan.csv
rsan
eng; US
McMurdo Dry Valleys LTER
rsan
Data Source Definition : Stream Transects - Anhydrobiosis of Nematodes - Data spreadsheet description, units used and column descriptions Record Delimiter : \r Number of Header Lines : 1 Number of Footer Lines : 1 Orientation : Column Quote Character :
"Field Delimiter : ,
false
LOCATION
Name of area where measurement was made
DATE_TIME
Date on which sample was gathered
Date Time Format: MM/DD/YYYY h:m:s
SAMPLE #
Sample ID
TYPE OF ORGANISM
Family associated with organism
SPECIES
Species found in soil
SEX
Gender of organism (male vs. female)
LIVE/DEAD/COMBINED
Survival Status (living, dead, or both)
ADULT/JUVENILE/COMBINED
Maturity (adult, juvenile, or both)
PERCENT COILED
Percentage of the nematodes found in a coiled state
Comments
Comments
FILE NAME
Name of file in which data was stored
DBF
https://mcm.lternet.edu/sites/default/files/data/rsan.csv
dataset
Approximately 1500g of soil or sediment were collected into Whirl-Pak(tm) bags with clean plastic scoops from 5 sections (0, 8, 16, 24, and 32 m away from the stream) along each transect. Five grams of sediment/soil were also collected from the top 1 cm for measurement of chlorophyll-a concentrations. Samples were mixed within the bags, placed into insulated coolers, and transported for analysis to the McMurdo laboratories. The soils were processed within 24 days of collection from the field. Until then, the soils were stored in a 4_C refrigerator. To extract the anhydrobiotic (coiled) nematodes, 100g of soil were placed into a beaker with 200mL 1.25M sugar solution. Then, a long spatula was used to stir the soil so that it was suspended in the sugar (30 seconds of stirring). Next, the sample was poured onto a set of No. 40 over No. 400 U.S.A. standard testing sieves that had been pre-wetted with 1.25M sugar solution. The top screen was rinsed with 1.25M sugar solution and then removed. The bottom screen was rinsed with 1.25M sugar solution that was squirted only on the front of the screen at an angle. Using this rinsing method, the soil was carefully worked to the bottom wedge of the screen, and the sugar volume was reduced by gently tapping the screen and letting more solution pass through. Next, the sample was rinsed into a 150mL beaker through a funnel, and then the funnel rinsed to catch any residue. Once the sample was collected into the 150mL beaker, the sugar and sediment was pipetted (using an automatic pipette) into a centrifuge tube containing 2M sugar solution. The pipetting was done slowly and at an angle so that the boundary between the two sugar layers was maintained. Then the beaker was rinsed with 1.25M sugar solution and the dregs were pipetted into the centrifuge tube as well. When necessary, more than one centrifuge tube was used to collect all the sample. Next, the tubes with sample were centrifuged for five minutes at 1744 RPM. Then, the liquid contents of the tube were poured through a No. 500 sieve. Finally, the contents of the sieve were rinsed into glass centrifuge tubes and stored at 4_C until counting. Samples were washed in to a counting dish and examined under a microscope at x100 or x200 magnification. Nematodes were identified as coiled or straight and counted. Total numbers in each sample were recorded on data sheets. Data were entered in to Excel files, printed, and checked for errors.
Metadata Access Constraints: none Metadata Use Constraints: none
annually
McMurdo Dry Valleys LTER
http://mcmlter.org/
pointOfContact