A total of 232 soils (0-5 cm depth) were collected from twelve ice-free areas along the Shackleton Glacier from December 2017 to January 2018. The locations include Roberts Massif, Schroeder Hill, Bennett Platform, Kitching Ridge, Mt. Augustana, Mt. Heekin, Thanksgiving Valley, Taylor Nunatak, Mt. Franke, Mt. Wasko, Nilsen Peak, and Mt. Speed, and range from 150 to 2221 m.a.s.l. in elevation. Between 14 and 26 soil samples were collected along elevation transects (up to 2000 m in length) from each location to capture maximum variation in soil properties, geochemistry, and surface exposure age.Nematodes, tardigrades and rotifers were extracted using a sugar centrifugation technique developed for Antarctic soils (Freckman & Virginia, 1993), and identified and enumerated via light microscopy. Mites and springtails were picked individually from each sample using a dissection microscope by mixing 50 g soil with 500 ml sugar solution (454 g L-1) and removing individual animals as they floated to the surface. However, microarthropods were depauperate such that they were not assessed in this study. Tardigrades and rotifers were identified to the phylum level, nematodes were identified to genus (Scottnema, Eudorylaimus, and Plectus) and as living or dead, life stage (juveniles or adults), and sex. Soil gravimetric moisture was measured by weighing 50 g subsamples before and after oven drying at 105°C for 24 h. Invertebrate abundances were assessed as the number of individual animals per kilogram of dry soil.Reference Cited: Freckman, D. W., & Virginia, R. A. (1993). Extraction of nematodes from Dry Valley Antarctic soils. Polar Biology, 13(7), 483–487. https://doi.org/10.1007/BF00233139